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KMID : 0525720000050010009
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Journal of Chitin and Chitosan
2000 Volume.5 No. 1 p.9 ~ p.14
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Changes in the Distribution and Activity of N-Acetyl-β-D-hexosaminidase and Chitinase During Cultivation of Soybean Sprouts
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Park RD
Cho KC/Jo YY/Kim KY/Jang GW/Shim JH/Kim YW/Suh YT
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Abstract
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During cultivation of soybean sprouts changes in distribution and activity of N-acety1- β -D-hexosaminidase and chitinnse, which are known as pathogenesis-related proteins, were studied. Content of water-extractable Protein was increased with the increase in fresh weight and length of soybean sprouts during cultivation. N-acety1- β -D-hexosaminidase and chitinase. distributed mainly in cotyledon, were increased with cultivation. Two endochitinase isoforms (34 and 54 kD) were detected in active staining.34 kD chitinase was drastically increased 4 days after germination, and 54 kD chitinase was detected only S and 6 days after germination.
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